Baobab (Adansonia digitata L., Malvaceae) seed oil contains Fatty Acids (FAs) with commercial and cosmeceutical value. The influence of provenance and geographical region on FAs profile and content of seed oil from baobab wild populations in sub-Saharan Africa (SSA) was assessed in this study. The FA profiles were determined as fatty acid methyl esters (FAME) by Gas Chromatography (GC). Generally, the seed oil contained 17–22% saturated fatty acids (SFA), 32–38% monounsaturated fatty acids (MUFA) and 22–26% polyunsaturated fatty acids (PUFA).