Chapter 7. Maize germplasm phenotyping for MLN, MCMV and SCMV under artificial inoculation at the MLN Screening Facility, Naivasha, Kenya

We describe here the protocols followed by CIMMYT at the MLN Screening Facility, Naivasha, Kenya, for culturing and increase of inoculum for MLN-causing viruses (MCMV and SCMV) in eastern Africa, followed by phenotyping of maize germplasm against MLN (under artificial inoculation with MCMV + SCMV) or for individual viruses (MCMV or SCMV) under controlled (nethouse) conditions.